Z700282
Sigma
Protein Sequencing Protocols, 2nd ed.
| ISBN-10: | 0-89603-975-7 |
| ISBN-13: | 978-0-89603-975-9 |
Description
| General description | This book is an update to the much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. This comprehensive book provides investigators a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination. |
Properties
| publication info | B.J. Smith, Humana Press, 2002, 508 pp., hard cover |
Table Of Contents
| Table of Contents | Strategies for Handling Polypeptides on a Micro-Scale SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization Enzymatic Cleavage of Proteins Chemical Cleavage of Polypeptides Enzymatic Preparation and Isolation of Glycopeptides Analytical and Micropreparative Capillary Electrophoresis of Peptides High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues Hydrolysis of Samples for Amino Acid Analysis Amino Acid Analysis: Precolumn Derivatization Methods Post Column Amino Acid Analysis Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection d-Amino Acid Analysis Validation of Amino Acid Analysis Methods Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis Identification of PTH-Amino Acids by HPLC Protein Sequencer Maintenance and Troubleshooting N-Terminal Protein Sequencing for Special Applications Identification of Phosphorylation Sites by Edman Degradation Validation of Protein Sequencing in a Regulated Laboratory Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method Chemical Modifications of Proteins as an Aid to Sequence Analysis Deblocking of N-Terminally Modified Proteins Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid Identification of Sites of Protein Glycosylation Analysis of Sites of Protein Phosphorylation Quantitation and Location of Disulfide Bonds in Proteins Getting the Most from Your Protein Sequence Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids Appendix 2: Protein Consensus Sequence Motifs Index |
